Evaluating the role of coevolution in a horizontally transmitted mutualism

Abstract

Mutualism depends on the alignment of host and symbiont fitness. Horizontal transmission can readily decouple fitness interests, yet horizontally transmitted mutualisms are common in nature. We hypothesized that pairwise coevolution and specialization in host-symbiont interactions underlies the maintenance of cooperation in a horizontally transmitted mutualism. Alternatively, we predicted selection by multiple host species may select for cooperative traits in a generalist symbiont through diffuse coevolution. We tested for signatures of pairwise coevolutionary change between the squash bug Anasa tristis and its horizontally acquired bacterial symbiont Caballeronia spp. by measuring local adaptation. We found no evidence for local adaptation between sympatric combinations of A. tristis squash bugs and Caballeronia spp. across their native geographic range. To test for diffuse coevolution, we performed reciprocal inoculations to test for specialization between three Anasa host species and Caballeronia spp. symbionts isolated from conspecific hosts. We observed no evidence of specialization across host species. Our results demonstrate generalist dynamics underlie the interaction between Anasa insect hosts and their Caballeronia spp. symbionts. Specifically, diffuse coevolution between multiple host species with a shared generalist symbiont may maintain cooperative traits despite horizontal transmission.