A microcalorimetric approach for investigating stoichiometric constraints on the standard metabolic rate of a small invertebrate

Abstract

1: Understanding the determinant of metabolism is a core ecological topic since it permits to link individuals energetic requirements to the ecology of communities and ecosystems. Yet, besides temperature, the effects of environmental factors on metabolism remain poorly understood. For example, dietary stoichiometric constraints have been hypothesized to increase maintenance metabolism of small invertebrates, yet experimental support remains scarce.\n\n2: Here, we used microcalorimetric heat flow measurements to determine the standard metabolic rate (SMR) of Daphnia magna throughout its ontogeny when fed stoichiometrically balanced (C/P ratio:166) or imbalanced (C/P ratio:1439) diets.\n\n3: When fed a stoichiometrically imbalanced diet, daphnids were able to maintain the stoichiometric homeostasis within narrow boundaries. However, they consistently increased their SMR while decreasing their somatic growth rate. Our measurements unequivocally demonstrate that homeostatic regulation implies higher metabolic costs and thereby reduces the portion of energy that can be allocated to growth.\n\n4: Our study demonstrates that microcalorimetry is a powerful and precise tool for measuring the metabolic rate of small-sized organisms and opens promising perspectives for understanding how environmental factors, such as nutritional constraints, affect organismal metabolism.