Free volume theory explains the unusual behavior of viscosity in a non-confluent tissue during morphogenesis

Abstract

A recent experiment on zebrafish blastoderm morphogenesis showed that the viscosity ({eta}) of a non-confluent embryonic tissue grows sharply until a critical cell packing fraction ({phi}S). The increase in{eta} up to{phi} S is similar to the behavior observed in several glass forming materials, which suggests that the cell dynamics is sluggish or glass-like. Surprisingly,{eta} is a constant above{phi} S. To determine the mechanism of this unusual dependence of{eta} on{phi} , we performed extensive simulations using an agent-based model of a dense non-confluent two-dimensional tissue. We show that polydispersity in the cell size, and the propensity of the cells to deform, results in the saturation of the available free area per cell beyond a critical packing fraction. Saturation in the free space not only explains the viscosity plateau above{phi} S but also provides a relationship between equilibrium geometrical packing to the dramatic increase in the relaxation dynamics.