Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile; Aidan M. Nikiforuk; Pawan K. Pandoh; David D. W. Twa; Duane E. Smailus; Jason Nguyen; Stephen Pleasance; Angus Wong; Yongjun Zhao; Diane L. Eisler; Michelle Moksa; Qi Cao; Marcus Wong; Edmund Su; Martin I. Krzywinski; Jessica Nelson; Andrew J. J. Mungall; Frankie Tsang; Leah Prentice; Agatha N. Jassem; Amee R. Manges; Steven J.M. Jones; Robin Coope; Natalie Prystajecky; Marco A. Marra; Mel Krajden; Martin Hirst

doi:10.1016/j.jviromet.2021.114339

Genetic & Genomic MedicineJournal of Virological MethodsOct 21, 2021

Peer-reviewed journal article

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile iD, Aidan M. Nikiforuk iD, Pawan K. Pandoh iD, David D. W. Twa iD, Duane E. Smailus iD, Jason Nguyen iD, Stephen Pleasance, Angus Wong iD, Yongjun Zhao iD, Diane L. Eisler, Michelle Moksa iD, Qi Cao iD, Marcus Wong iD, Edmund Su iD, Martin I. Krzywinski iD, Jessica Nelson iD, Andrew J. J. Mungall iD, Frankie Tsang iD, Leah Prentice iD, Agatha N. Jassem iD, Amee R. Manges iD, Steven J.M. Jones iD, Robin Coope iD, Natalie Prystajecky iD, Marco A. Marra iD, , Martin Hirst iD

Abstract

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity. Article SummaryA scalable, non-proprietary, magnetic bead-based automated nucleic acid extraction protocol optimised for minimum cross-well contamination

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