Durable Antimicrobial Microstructure Surface (DAMS) Enabled by 3D-Printing and ZnO Nanoflowers

A.Numerous studies have been trying to create nanomaterials based antimicrobial surfaces to combat the growing bacterial infection problems. Mechanical durability has become one of the major challenges to applying those surfaces in real life. In this study, we demonstrate the Durable Antimicrobial Microstructures Surface (DAMS) consisting of DLP 3D printed microstructures and zinc oxide (ZnO) nanoflowers. The microstructures serve as a protection armor for the nanoflowers during abrasion. The antimicrobial ability was tested by immersing in 2E8 CFU/mL Escherichia coli (E. coli) suspension and then evaluated using electron microscopy. Compared to the bare control, our results show that the DAMS reduces bacterial coverage by more than 90% after 12 hrs of incubation and approximately 50% after 48 hrs of incubation before abrasion. Importantly, bacterial coverage is reduced by approximately 50% after 2 min of abrasion with a tribometer, and DAMS remains effective even after 6 min of abrasion. These findings highlight the potential of DAMS as an affordable, scalable, and durable antimicrobial surface for various biomedical applications.