Dynamic changes in the plasmidome and resistome in the gastrointestinal tract of chickens

The expansion of intensive poultry farming has led to a substantial increase in antibiotic use, which in turn has promoted the accumulation of antibiotic resistance genes (ARGs). The chicken gut serves as a reservoir for these genes and provides favorable conditions for their horizontal transfer via mobile genetic elements, such as plasmids. Through this process, commensal bacteria can transfer ARGs to pathogens, facilitating their spread and increasing the risk of transmission to humans. In this study, long-read sequencing was used to characterize plasmidome and resistome in 12 fecal samples from three houses of a commercial chicken broiler farm. All chickens received enrofloxacin in the first days of life, with one house additionally treated with sulfamethoxazole/trimethoprim combination. For comparison, metagenomic analysis using short-read sequencing was performed on the same samples. This study revealed the presence of various ARGs associated with resistance to 26 antibiotic classes. Strong genetic association between MOBP-type plasmids and fluoroquinolone resistance was observed within chicken broiler farm. Temporal trends indicated progressive mobilization of these ARGs, suggesting an increasing potential for horizontal gene transfer. While fluoroquinolone resistance expanded over time, diaminopyrimidine resistance remained stable despite the antibiotic treatment. Most ARGs were carried on small plasmids, and complete plasmid reconstructions ranged from 2.6 to 47.6 kb. Despite technical limitations, our findings demonstrate that plasmidome sequencing can enrich metagenomic analysis by enabling the detection of low-abundance plasmid types and providing deeper insights into the dynamic plasmid-mediated dissemination of ARGs in the chicken gut microbiome. ImportanceDespite the crucial role of plasmids in antimicrobial resistance (AMR) dissemination, studies focusing on plasmidome, defined as the complete set of plasmids, remain limited. Combining a metagenomic approach with a focus on plasmids enhances our ability to understand the genetic context and mechanisms underlying AMR transmission. The findings emphasize the importance of targeted plasmid analysis to improve surveillance and risk assessment of AMR transmission in microbial ecosystems.