Developing A Programmable, Self-Assembling Squash Leaf Curl China Virus (SLCCNV) Capsid Proteins Into \"Nano-Cargo\"-Like Architecture: A Next-Generation \"Nanotool\" For Biomedical Applications

A new era has begun in which pathogens have become useful scaffolds for nanotechnology applications. In this research/study, an attempt has been made to generate an empty cargo-like architecture from a high-profile plant pathogen of Squash leaf curl China virus (SLCCNV). In this approach, SLCCNV coat protein monomers are obtained efficiently by using a yeast Pichia pastoris expression system. Further, dialysis of purified SLCCNV-CP monomers against various pH strengthenened (5-10) disassembly and assembly buffers produced a self-assembled \"Nanocargo\"-like architecture, which also exhibited an ability to encapsulate the magnetic nanoparticles at in vitro. Bioinformatics tools were also utilized to predict the possible self-assembly kinetics and bioconjugation sites as well. The biocompatibility of \"SLCNNV-CP-Nanocargo\" particles was also evaluated by in vitro cancer cells, which eventually proved the particles to be versatile material for the next generation \"nanotool\" capable of housing various therapeutic or imaging agents.