Tobias W. Giessen

This paper introduces a permeable protein nanocage, QtEnc, enabling rapid and efficient cytosolic protein delivery through post-assembly cargo loading and pH-triggered release.

This study identifies a two-component Family 2B encapsulin shell that compartmentalizes geosmin synthase, showing its role in enzyme activity and stability through cargo loading peptides and pore conformation changes.

The paper tests whether the Kuenenia stuttgartiensis encapsulin protects against NO damage and finds no evidence of such protection.

This paper introduces a directed evolution strategy for multimeric proteins using gene duplication to enable the selection of diverse high-performing variants.

This study shows that the McdAB system can be used to engineer spatial control of various bacterial organelles in E. coli, enabling programmable positioning for synthetic biology applications.

The paper characterizes a two-component encapsulin from Streptomyces lydicus, showing differential assembly and mixed shell formation with irregular tiling.

The paper characterizes a DyP encapsulin from enterobacteria, showing its structural features, stability under harsh conditions, and catalytic activity preference at low pH.

The paper redefines the function of EshA as a protein shell encapsulating 2-MIB synthase, showing it is a cargo-loaded nanocompartment for secondary metabolite biosynthesis.

This paper introduces a pore-engineered enzyme nanoreactor platform based on encapsulins to improve catalytic activity and substrate range.

This study characterizes the structure and biochemical function of an Acinetobacter baumannii RLD, showing its sulfurtransferase activity and potential ternary catalytic mechanism.

The paper shows that a single point mutation in a virus-like capsid can drive the formation of tetrahedral structures instead of the typical icosahedral symmetry.

This paper engineers protein nanocages to package both RNA and protein in vivo, demonstrating their ability to self-assemble and deliver multiple functional RNAs along with proteins.

This study investigates how pore structure affects stability and molecular flux in engineered protein cages through design, structural analysis, and simulations.

This study engineers a protein nanocage that can reversibly disassemble and reassemble under physiological conditions, enabling controlled cargo encapsulation.

The paper engineers inter-species amino acid cross-feeding in bacterial consortia to increase population evenness in synthetic microbial communities.

This paper describes an engineered E. coli system that senses Candida albicans via HPA and inhibits hypha formation to reduce virulence.

This study characterizes a bacterial iron storage compartment using structural methods, revealing its large iron storage capacity and functional mechanism.

This paper demonstrates the use of prokaryotic encapsulins to create synthetic organelles in yeast, enabling compartmentalization of proteins and enzymatic activity.

The paper identifies and characterizes encapsulin nanocompartments in prokaryotes, revealing their role in sequestering toxic reactions and their involvement in various metabolic processes.

The paper engineers genetically-encoded magnetism and mineralization by mutating ferritin and characterizing DUF892 family proteins in E. coli.