Kenya Tajima

This study evolves an artificial nucleocapsid from a circularly permuted bacterial protein cage, revealing heterogeneous cage architectures with distinct subunit arrangements and conformations.

The paper optimizes cell-free gene expression in microdroplets to enhance protein yield from single-copy DNA templates, achieving ~94 nM expression from ~0.12 pM DNA.

This paper develops a system for in vitro cargo-directed assembly of nonviral nucleocapsids with controlled size and shape, demonstrating encapsulation of various cargo molecules and morphological changes induced by DNA origamis.

This paper develops a cell-free directed evolution platform using droplet microfluidics to rapidly evolve RNA polymerases with new functions and applications.