bioRxivBiophysicsPreprint
Serialized On-grid Lift-In Sectioning for Tomography (SOLIST)
P. S. Erdmann, T.-D. H. Nguyen, G. Perone, R. Vazzana, F. K. Don, M. Silva, S. Sorrentino, P. Swuec, F. Leroux, N. Kalebic, F. Coscia
Abstract
Cryo-focused ion beam milling has enabled groundbreaking structural discoveries in native cells. Progress toward medically relevant applications, however, has been slow. We here present an adaptation of the cryo-lift out procedure for Serialized On-grid Lift-In Sectioning for Tomography (SOLIST), which increases throughput, reduces ice contamination, and enhances sample stability. With these improvements, new specimens, ranging from high-pressure frozen reconstituted LLPS droplets to human forebrain organoids, are accessible to cryo-electron tomography.
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