J. M. Plitzko

In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography

This study uses cryo-ET to visualize copia retrotransposon structure in Drosophila cells, revealing nuclear clustering and size selection during gametogenesis.

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.

Serial Lift-Out - Sampling the Molecular Anatomy of Whole Organisms

This paper introduces Serial Lift-Out, a technique that improves sample preparation for cryo-ET, enabling the study of multicellular molecular anatomy in C. elegans.

Cryo-plasma FIB/SEM volume imaging of biological specimens

This paper introduces cryo-plasma FIB/SEM for high-resolution 3D imaging of biological samples, achieving resolutions of 20-50 nm and enabling in-situ structural biology applications.

In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

The paper visualizes the native structure of the ciliary transition zone and the stepwise assembly of IFT trains in Chlamydomonas cells.

Determinants shaping the nanoscale architecture of the mouse rod outer segment

This study uses cryo-ET to reveal molecular connectors and supramolecular assemblies in mouse rod outer segments, explaining their structural organization and linking them to genetic diseases.

A Modular Platform for Streamlining Automated Cryo-FIB Workflows

The paper introduces SerialFIB, an open-source software tool for automating cryo-FIB workflows, enabling customizable and efficient lamella preparation for cryo-electron tomography.

Molecular-scale visualization of sarcomere contraction within native cardiomyocytes

This study uses cryo-electron tomography to visualize sarcomere contraction in neonatal rat cardiomyocytes, revealing thin filament sliding and structural changes during muscle contraction.

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity

This study determines the structure of cyanobacterial VIPP1 rings using cryo-EM, revealing how VIPP1 oligomerization and lipid binding maintain thylakoid membrane integrity.

Charting the native architecture of thylakoid membranes with single-molecule precision

The paper uses cryo-electron tomography to map the native molecular landscape of thylakoid membranes in green algae, revealing segregation of photosystems I and II at membrane domain borders.

Reconstitution of a flexible SYCP3-DNA fibre suggests a mechanism for SYCP3 coating of the meiotic chromosome axis

This study uses cryo-electron tomography and atomic force microscopy to show that SYCP3 forms a flexible fibre that binds DNA, suggesting a mechanism for coating the meiotic chromosome axis.

In situ architecture of the algal nuclear pore complex

This study reports the in situ architecture of the algal nuclear pore complex in Chlamydomonas reinhardtii and compares it to the human NPC, revealing structural variations between species.