bioRxivScientific Communication & EducationPreprint
Gelato streamlines reproducible and auditable assembly of Western blot figures
Abstract
Western blotting has been widely used for decades, yet figure preparation remains unstandardized, error-prone and difficult to audit. Gelato is a free Fiji/ImageJ plugin that streamlines intuitive figure preparation, logs processing coordinates, provides a straightforward audit platform and enables reproduction from raw images. By making these capabilities simple and accessible, Gelato facilitates robust Western blot reporting, reducing a substantial burden on authors and reviewers while strengthening scientific rigor.
- Citations
- 0
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Blind spots on western blots: a meta-research study highlighting opportunities to improve figures and methods reporting
C. Kroon, L. Breuer, … T. L. Weissgerber·bioRxiv·May 13, 2022
- GelBox: Open-source software to improve rigor and reproducibility when analyzing gels and immunoblots
U. Gulbulak, A. G. Wellette-Hunsucker, K. Campbell·bioRxiv·Mar 7, 2024
- Improving rigor and reproducibility in western blot experiments with the blotRig analysis software
Cleopa Omondi, Austin Chou, … Adam R. Ferguson·Scientific ReportsPeer-reviewed·Sep 17, 2024
- Emerging Concern of Scientific Fraud: Deep Learning and Image Manipulation
- A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting
Marc R. Birtwistle, Jonah R. Huggins, … Delphine Dean·ACS OmegaPeer-reviewed·Mar 4, 2025
- Mesowestern Blot: Simultaneous Analysis of Hundreds of Sub-Microliter Lysates
C. O. Zadeh, J. R. Huggins, … M. R. Birtwistle·bioRxiv·Nov 7, 2021