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bioRxivBioinformaticsPreprint

m6ASeqTools: An R toolkit for post-processing m6A sites detected by m6Anet

Hanna LeeiD, Denalda GashiiD, Syeda Maheen BatooliD, Ana K. EscobedoiD, Bob S. CarteriD, Leonora BalajiD

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Abstract

m6ASeqTools is an R package designed to streamline the post-processing and interpretation of site-level m6A predictions from m6Anet. It provides descriptive summaries of m6A distribution across transcripts, genes, biotypes, and transcript regions, and enables condition comparisons through a calculated weighted modification ratio. By integrating differential gene expression data, the package links methylation changes with expression differences, providing biotype-specific and region-specific insights into how m6A localization patterns relate to transcriptional regulation. Availability and implementationm6ASeqTools is freely available at https://github.com/hannalee809/m6ASeqTools Supplementary informationSupplementary data are available at Bioinformatics online.

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