Accelerated Nitrogen Cycling on Seagrass Leaves in a High-CO2 World

Seagrass meadows form highly productive and diverse ecosystems in coastal areas worldwide, where they are increasingly exposed to ocean acidification (OA). Efficient nitrogen (N) cycling and uptake are essential to maintain plant productivity, but the effects of OA on N transformations in these systems are poorly understood. Here we show that complete N cycling occurs on leaves of the Mediterranean seagrass Posidonia oceanica, with OA affecting both N gain and loss while the prokaryotic community structure remains largely unaffected. Daily leaf-associated N2 fixation contributed to 35% of the plants N demand under ambient pH, whereas it contributed to 45% under OA. Nitrification potential was only detected under OA, and N-loss via N2 production increased, although the balance remained decisively in favor of enhanced N gain. Our work highlights the role of the N-cycling microbiome in seagrass adaptation to OA, with key N transformations accelerating towards increased N gain.