H. Huang

A single-cell transcriptomic atlas of the pigtail macaque lung

This paper constructs a single-cell transcriptomic atlas of the pigtail macaque lung, revealing high similarity to human lung cell types and providing a reference for studying respiratory diseases in a translational model.

ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota

This study shows ASB3 worsens inflammatory bowel disease by destabilizing TRAF6 and altering gut microbiota, suggesting ASB3 inhibition could be a therapeutic strategy.

A cross ancestry genetic study of psychiatric disorders from India

This study evaluates the predictive accuracy of polygenic scores for psychiatric disorders in an Indian population using European GWAS data, finding moderate-to-high accuracy for bipolar disorder but lower for OCD and externalizing traits.

TET2 regulates early and late transitions in exhausted CD8+ T-cell differentiation and limits CAR T-cell function

This study shows that targeting TET2 in CAR T-cells improves their anti-tumor responses by limiting terminal exhaustion and enhancing therapeutic potential.

Loss of an extensive ciliary connectome induces proteostasis and cell fate switching in a severe motile ciliopathy

The paper identifies cellular defects beyond motility loss in ciliopathies caused by CCDC39/CCDC40 variants, showing proteostasis disruption and cell fate switching.

An optimized somatic embryo transformation system assisted homozygous edited rubber tree generation method mediated by CRISPR/Cas9

This study develops a CRISPR/Cas9-based method to generate homozygous edited rubber tree plants by optimizing somatic embryo transformation and subculture procedures.

Thymidine Phosphorylase Promotes the Formation of Abdominal Aortic Aneurysm in Mice Fed a Western Diet

This study shows that TYMP promotes abdominal aortic aneurysm (AAA) in mice fed a Western diet by enhancing inflammation, thrombosis, and MMP2 activation while inhibiting vascular smooth muscle cell function.

Neurotensin-Neurotensin Receptor 2 signaling in adipocytes regulates food intake through ceramide metabolism

This study shows that NTS-NTSR2 signaling in white adipocytes regulates food intake by modulating ceramide metabolism and GDF15 production.

The stability of lower genital tract microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

This study investigates the relationship between lower genital tract microbiota and reproductive outcomes in PCOS patients, finding that higher Lactobacillus abundance correlates with better IVF-FET outcomes.

Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation

The paper identifies mitochondrial reverse electron transport in myeloid cells as a driver of neuroinflammation and shows that blocking it protects the CNS in disease models.

E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation

This study identifies ASB3 as an E3 ligase that suppresses antiviral immunity by ubiquitinating and degrading MAVS, thereby inhibiting IFN-I responses and promoting viral replication.

AhR ligands from LGG metabolites promote piglet intestinal ILC3 activation and IL-22 secretion to inhibit PEDV infection

This study shows LGG metabolites activate ILC3 cells via AhR to produce IL-22, which prevents PEDV infection in piglets by enhancing intestinal cell proliferation and STAT3 signaling.

Unstructured Domains in Assembly Factors Promote the Hierarchy of Ribosome Maturation via Their Structural Plasticity.

The paper investigates how the non-globular structure of the assembly factor Ltv1 uses structural plasticity to coordinate 40S ribosome subunit maturation and reveals its role in quality control and human disease.

Quantitative Three-dimensional Label-free Digital Holographic Imaging of Cardiomyocyte Size, Ploidy, and Cell Division

This study uses digital holographic imaging to show that mononucleated cardiomyocytes retain proliferative potential while binucleated cells have limited capacity to divide, with size thresholds affecting division ability.

Germline cis variant determines epigenetic regulation of the anti-cancer drug metabolism gene dihydropyrimidine dehydrogenase (DPYD)

The paper identifies a germline variant that regulates DPYD gene expression through an enhancer, affecting 5-FU drug resistance.

MiceVAPORDot: A novel automated approach for high-throughput behavioral characterization during E-cigarette exposure in mice

MiceVAPORDot automates behavioral analysis during e-cigarette exposure in mice, revealing phenotypes not detectable by traditional methods.

Yes1-mediated Cul9 phosphorylation promotes the metabolic reprogramming in gastric cancer

This study identifies Yes1-mediated Cul9 phosphorylation as a driver of metabolic reprogramming in gastric cancer, revealing a feedback loop with therapeutic implications.

Nuclear speckles regulate HIF-2α programs and correlate with patient survival in kidney cancer

This study shows nuclear speckles vary in cancer, correlate with survival in kidney cancer, and are regulated by HIF-2 through speckle targeting motifs.

Genetically engineered mesenchymal stem cells as a nitric oxide reservoir for acute kidney injury therapy

This paper genetically engineers mesenchymal stem cells to act as a nitric oxide reservoir, enhancing therapeutic efficiency in acute kidney injury by precise spatiotemporal NO release.

Epigenetic regulation of p63 blocks squamous-to-neuroendocrine transdifferentiation in esophageal development and malignancy

This study shows that p63 regulates the balance between squamous and neuroendocrine cell fates in esophageal development and cancer by modulating epigenetic mechanisms.

An end-to-end workflow for non-destructive 3D pathology

This paper provides an end-to-end workflow for non-destructive 3D pathology using light-sheet microscopy, focusing on fluorescent H&E analogs for preclinical and clinical studies.

Extracting herbicide and antibiotic natural products from a plant-microbe interaction system

This study identifies a plant-microbe interaction system where a rhizosphere bacterium produces molecules with dual antibiotic and herbicide functions, offering insights into novel natural product discovery.

Disrupted memory T cell expansion in HIV-exposed uninfected infants is preceded by premature skewing of T cell receptor clonality.

This study shows that HIV/ARV-exposed uninfected infants have reduced T cell receptor diversity and altered immune development, increasing infection risk.

Multiscale Model for Ion Transport in Cellular Media and Applications in Smooth Muscle Cells

This paper develops a multiscale model for ion transport in multicellular tissues and applies it to study smooth muscle cell activation under various stimuli, showing the importance of gap junctions and extracellular potassium.

In Vitro Modeling of CD8 T Cell Exhaustion Enables CRISPR Screening to Reveal a Role for BHLHE40

The paper establishes an in vitro model of CD8 T cell exhaustion and uses CRISPR screening to identify BHLHE40 as a regulator of T cell exhaustion differentiation.

Cyanobacteria using urea as a nitrogen source can overcome acid stress

This study shows that marine cyanobacteria using urea as a nitrogen source can better adapt to acid stress, with urease playing a key role in this adaptation.

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses

The paper identifies acetate as a novel energy stress marker produced in the liver via ACOT12/8-mediated conversion of acetyl-CoA from fatty acids, supporting its role in brain fueling and CoA recycling.

Patient and cell-type specific hiPSC-modeling of a truncating titin variant associated with atrial fibrillation

This study uses patient-derived iPSC-CMs to show that a titin truncating variant increases arrhythmia susceptibility and disrupts sarcomere structure in both atrial and ventricular tissues.

Single-cell transcriptional analysis of lamina propria lymphocytes in the jejunum reveals ILC-like cells in pigs

The paper identifies and characterizes ILC-like cells in porcine jejunum using scRNA-seq and flow cytometry, revealing subtype classifications and shared features with human ILCs.

StereoSiTE: A framework to spatially and quantitatively profile the cellular neighborhood organized iTME

StereoSiTE analyzes spatial transcriptomics data to identify cell-cell interactions in the tumor microenvironment and reveals neutrophil-driven communication networks in treated xenograft models.