A minimally invasive EEG recording method in mice using thin needle electrodes

Abstract

Currently, implantation of electroencephalogram (EEG) electrodes in laboratory animals is time-consuming and requires specialized equipment. We present a novel method for EEG recordings in mice that utilizes thin needle electrodes. These electrodes are inserted into the skull at predetermined locations by gently pressing them against the bone surface. To ensure stable fixation of the implant, hook-shaped needles are positioned along the lateral aspects of the skull. The electrodes are connected to a multipin connector and secured to the skull using dental composite, after which the animal is allowed to recover from anesthesia. Importantly, procedures such as skull drilling and screw placement are not required, allowing the entire surgery to be completed in less than 15 minutes. Consequently, this EEG implantation approach is rapid and minimally invasive. Results of our studies indicate that EEG recordings obtained with needle electrodes are not inferior to those obtained with screw electrodes. Overall, the method is designed to enhance the accuracy and efficiency of EEG recording studies while improving animal welfare. O_LISimplifies the placement of EEG electrodes. C_LIO_LIReduces the time required for electrode implantation. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=67 SRC="FIGDIR/small/715731v1_ufig1.gif" ALT="Figure 1"> View larger version (44K): org.highwire.dtl.DTLVardef@e5608org.highwire.dtl.DTLVardef@1325ea4org.highwire.dtl.DTLVardef@1e37202org.highwire.dtl.DTLVardef@1521bb8_HPS_FORMAT_FIGEXP M_FIG C_FIG