bioRxivBiophysicsPreprint
Wide-Field Multicontrast Nonlinear Microscopy for Histopathology
L. Uribe Casta\~no, K. Mirsanaye, A. Golaraei, L. Kontenis, S. J. Done, V. Stambolic, M. Akens, B. Wilson, V. Barzda
Abstract
A multicontrast polarimetric wide-field second harmonic generation (SHG) and multiphoton excitation fluorescence (MPF) microscope is optimized for large area imaging of hematoxylin and eosin (H&E) stained and unstained histology slides. The bleaching kinetics of MPF and SHG are examined with various laser intensities at different pulse repetition rates to determine the optimal wide-filed imaging conditions for H&E stained histology slides. Several polarimetric parameters are used to investigate the organization of extracellular matrix collagen in the histology samples.
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