Improved chromosome level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) based on SMRT Sequencing and linkage map.

Daniel Blande; Olli‐Pekka Smolander; Virpi Ahola; Pasi M. A. Rastas; Jaakko Tanskanen; Juhana Kammonen; Vicencio Oostra; Lorenzo Pellegrini; Suvi Ikonen; Tad Dallas; Michelle F. DiLeo; Anne Duplouy; Ilhan Cem Duru; Pauliina Halimaa; Aapo Kahilainen; Suyog S. Kuwar; Sirpa O Kärenlampi; Elvira Lafuente; Shiqi Luo; Jenny Makkonen; Abhilash Nair; Maria de la Paz Celorio‐Mancera; Ville Pennanen; Annukka Ruokolainen; Tarja Sundell; Arja I. Tervahauta; Victoria Twort; Erik van Bergen; Janina Österman; Lars G. Paulin

doi:10.1101/2020.11.03.364950

GenomicsbioRxivNov 3, 2020

Preprint

Improved chromosome level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) based on SMRT Sequencing and linkage map.

Daniel Blande iD, Olli‐Pekka Smolander iD, Virpi Ahola iD, Pasi M. A. Rastas iD, Jaakko Tanskanen iD, Juhana Kammonen iD, Vicencio Oostra iD, Lorenzo Pellegrini iD, Suvi Ikonen, Tad Dallas iD, Michelle F. DiLeo iD, Anne Duplouy iD, Ilhan Cem Duru iD, Pauliina Halimaa iD, Aapo Kahilainen iD, Suyog S. Kuwar iD, Sirpa O Kärenlampi, Elvira Lafuente iD, Shiqi Luo iD, Jenny Makkonen iD, Abhilash Nair iD, Maria de la Paz Celorio‐Mancera iD, Ville Pennanen iD, Annukka Ruokolainen, Tarja Sundell iD, , Marjo Saastamoinen iD

Abstract

The Glanville fritillary (Melitaea cinxia) butterfly is a long-term model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome level assembly of the butterflys genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses. The final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO, indicates that the genome contains 93 - 95% of the BUSCO genes in complete and single copies. We predicted 14,830 gene models using the MAKER pipeline and manually curated 1,232 of these gene models. The genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome and genetics studies on this species.

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