The interplay of HSP90s with YDA regulates main body axis formation during early embryogenesis in Arabidopsis

Abstract

The YODA kinase (YDA) pathway is intimately associated with the control of Arabidopsis thaliana embryo development but little is known regarding its regulators. Using genetic analysis, HEAT SHOCK PROTEINS 90 (HSP90s) emerge as potent regulators of YDA in the process of embryo development and patterning. This study is focused on the characterization and quantification of early embryonal traits of single and double hsp90 and yda mutants. The mutant analysis was supported by expression analyses of cell-specific WUSCHEL-RELATED HOMEOBOX 2 (WOX2) and WOX8 genes during early embryonic development. Chromatin immunoprecipitation assays corroborated the involvement of YDA and HSP90s in the epigenetic control of chromatin remodeling during early embryogenesis. Genetic interactions among HSP90s and members of the YDA signaling pathway affected the development of both embryo proper and suspensor. Impaired function of HSP90s or YDA had an impact on the spatiotemporal expression of WOX8 and WOX2 suggesting their essential role in cell fate determination and interference with auxin distribution. Hence, the interplay between HSP90s and YDA signaling cascade mediates the epigenetic control regulating the transcriptional networks shaping early embryo development.