Biosynthesis and heterologous expression of cacaoidin, the first member of the lanthidin family of RiPPS

1.Cacaoidin is the first member of the new lanthidin RiPP family, a lanthipeptide produced by the strain Streptomyces cacaoi CA-170360 with unprecedented features such as an unusually high number of D-amino acids, a double methylation in the N-terminal alanine and a tyrosine residue glycosylated with a disaccharide. In this work, we describe the complete identification, cloning and heterologous expression of the cacaoidin biosynthetic gene cluster, which shows unique RiPP genes.