Capillary bundling of microtubules by condensates

Abstract

The cytoskeleton organizes the cellular interior using cytoskeletal filaments that rely on bundling, usually executed by stable and ordered crosslinking proteins. Bundling often requires protein complexes with at least two defined microtubule binding regions, as present in many molecular motors. Here, we establish a mechanism of microtubule bundling based on capillary forces, analogous to how wet hair sticks together. We show using in vitro experiments and theory that condensates can bundle microtubules through capillary forces, wherein liquid-like capillary bridges form between microtubules and adhere them together through interfacial and wetting forces. We quantify the structure and dynamics of these capillary bundles using total internal reflection fluorescence microscopy, and directly measure the charge-dependent interfacial tensions of condensates on microtubules using atomic force microscopy. Lastly, we show that these capillary bridges provide viscous resistance to motor-driven microtubule sliding that is insensitive to the bulk protein concentration. Taken together, we provide a novel mechanism for how cytoskeletal filaments bundle: through condensate-mediated capillary forces.