Bending and Squeezing: Gradual Potentials Encode Mechanical Stimuli in Poplar

Abstract

Mechanical stimuli such as wind elicit rapid electrical signals in plants, yet the mechanisms driving these responses remain poorly understood. Here, we investigated the electrophysiological responses of young poplar trees to controlled stem bending. We identified a gradual potential (GP) distinct from classical action potentials, whose amplitude attenuation and propagation distance were strongly influenced by stimulus speed and intensity. Although maximal GP amplitude near the bending site remained stable across stimulation setups, slower and gentler flexions led to more rapid spatial decay and reduced signal propagation. Similar GP responses were observed following either stem bending or direct root pressurization, suggesting hydraulic-electrical coupling. These results suggest that mechanical stress induces a transient hydraulic pressure wave that triggers the GP. While the GPs attenuation and propagation dynamics resemble those of a diffusive pressure signal, key features--such as constant peak amplitude regardless of stimulus strength and progressive signal narrowing--point to a complex, non-linear transduction mechanism. Altogether, our findings reveal a finely tuned system where spatial and temporal dynamics of this non-action potential electrical signal encode detailed information about mechanical stimuli, potentially allowing plants to adaptively respond to fluctuating environmental forces like wind. HighlightRoot pressurization generates electrical signals similar to stem bending in poplar, suggesting hydroelectric coupling in plant mechanosensing and wind intensity encoding.