A quinoa-associated Pantoea isolate displays salinity-responsive auxin production and promotes plant growth under salt stress

Abstract

Plant-associated bacteria can promote plant growth under saline conditions, but salinity-dependent changes in bacterial physiological traits remain insufficiently understood. Here, we isolated bacteria from seedlings of quinoa (Chenopodium quinoa Willd.) lines maintained under laboratory propagation for more than 30 years and evaluated their activity under saline conditions. A quinoa-associated Pantoea isolate, strain 6PN, promoted primary root elongation and whole-plant dry weight of Arabidopsis thaliana under salt stress, whereas no significant effect was observed under non-saline conditions. Comparative analyses with reference Pantoea agglomerans strains showed that strain 6PN exhibited salinity-responsive indole-3-acetic acid (IAA) production. Genome analysis identified a putative ipdC gene and additional genes related to stress responses, nutrient acquisition, polysaccharide biosynthesis and export, flagellar biosynthesis, and chemotaxis. Phylogenomic analysis indicated that strain 6PN was genomically distinct from representative Pantoea species examined here. In an Arabidopsis trench-plate assay, GFP-labeled strain 6PN was recovered from spatially separated plant tissues at higher levels than a GFP-labeled reference strain under saline conditions. These results identify strain 6PN as a quinoa-associated Pantoea isolate with salinity-responsive IAA production and plant growth-promoting activity under defined salt-stress conditions.