Functional Characterisation of Recombinant Proteins Using Ion Channel Switch Technology: A Label-Free, Wash-Free Platform for Biotechnological Applications

Abstract

We present a rapid, label-free, and highly sensitive platform for characterising recombinant protein functionality using Ion Channel Switch (ICS) technology. This method enables precise evaluation of binding specificity, oligomeric state discrimination, and real-time analyte detection, addressing key challenges in protein engineering and bioprocess quality control. Using engineered single-chain variable fragments (scFv), ICS reliably distinguishes monomeric from multimeric forms, facilitates wash-free detection of analytes ranging from small molecules to larger biomolecules, and enables quantitative biosensing within seconds in real-time and continuous format. These capabilities establish ICS as a powerful tool for streamlining recombinant protein screening, with broad applications in diagnostics, therapeutic quality control, and automated bioprocess workflows.