Investigation of oxidative processes and revealing the changes in purine nucleoside phosphorylase activity levels in model animals in the development of diabetes mellitus

Abstract

Diabetes mellitus is a global and one of the most common healthcare problems. The immune system, having a certain autonomy in the recognition and removal of foreign cells, antigens and other compounds is also under strict homeostatic control, in which a lot of/ variety of biochemical reactions take part. And since the problem of treatment of diabetes mellitus is still not solved, it requires detailed research of different elements of pathogenesis at the molecular level. Taking into account the fact that the connection/ link between immune processes and enzymes of purine metabolism is doubtless, it is of interest to investigate the changes in purine metabolism. In previous studies a frequent combination and interrelation of purine and carbohydrate metabolism was shown. Aim of this study was the investigation of the enzymatic activity of PNP in serum, liver and in pancreas, and also determination of levels of lipid peroxidation indicators, NO content in blood plasma, glutathione peroxidase activity in the mitochondrial fraction of the pancreatic tissue of white rats with modeled alloxan diabetes. The obtained results have demonstrated statistically significant changes in the PNP activity level indicators in the investigated organs and stages of the disease. The indicators of oxidative processes MDA, NO and changes of the activity of GPx and PNP can be used as additional paraclinical indicators for early diagnostics and for detection and refinement of the level of activity of the pathologic process, its development and stage of the disease, which contributes to the appointment of individualized adequate therapy.