bioRxivMicrobiologyPreprint
Bacterial efflux pumps excrete SYTO-TM dyes from bacteria and lead to false-negative staining results
Abstract
Multidrug efflux pumps excrete a range of small molecules from bacterial cells. In this study, we show that bacterial efflux pumps have affinity for a range of SYTO dyes that are commonly used to label bacteria. Efflux pump activity will there lead to false negative results from bacterial staining and SYTO dyes should be used with caution on live samples. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=94 SRC="FIGDIR/small/560001v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@1b8bab4org.highwire.dtl.DTLVardef@e9a84forg.highwire.dtl.DTLVardef@291058org.highwire.dtl.DTLVardef@1f0245d_HPS_FORMAT_FIGEXP M_FIG C_FIG
- organism: bacteria (other)
- assay: imaging
- research focus: host-pathogen & immune response
- approach: experimental assay
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A palette of fluorophores that are differentially accumulated by wild-type and mutant strains of Escherichia coli: surrogate ligands for bacterial membrane transporters
J. E. Salcedo Sora, S. Jindal, … D. B. Kell·bioRxiv·Jun 15, 2020
- Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli
- A guide for membrane potential measurements in Gram-negative bacteria using voltage-sensitive dyes
J. A. Buttress, M. Halte, … H. Strahl·bioRxiv·Apr 30, 2022
- Efflux pumps mediate changes to fundamental bacterial physiology via membrane potential
E. E. Whittle, O. Orababa, … T. W. Overton·bioRxiv·Apr 3, 2023
- Effect of 2,4-Dinitrophenol Against Multidrug-Resistant Bacteria
Pérez Carrillo Víctor Hugo, Nuñez Vázquez Ángel Alfredo, … Montiel Aguirre Jesús Fernando·bioRxiv·May 13, 2024
- Efflux only impacts drug accumulation in actively growing cells
E. Whittle, H. McNeil, … J. M. A. Blair·bioRxiv·May 11, 2021