bioRxivMicrobiologyPreprint
Chitin degradation by Synechococcus WH7803
G. Capovilla, K. G. Castro, S. Collani, S. M. Kearney, D. M. Kehoe, S. W. Chisholm
Abstract
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures - yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.
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