B. A. Barad

Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection

This study improves cryo-EM data collection by reducing resolution attenuation during stage tilt, enabling isotropic map reconstruction of specimens with preferred orientations.

Mapping Protein Dynamics at High-Resolution with Temperature-Jump X-ray Crystallography

This study uses temperature-jump X-ray crystallography to observe atomic-scale protein motions in lysozyme, showing how vibrations evolve into functional movements and how inhibitors alter these dynamics.

A surface morphometrics toolkit to quantify organellar membrane ultrastructure using cryo-electron tomography

The paper introduces a surface morphometrics toolkit for quantifying organellar membrane ultrastructure using cryo-ET, showing ER stress induces adaptive mitochondrial ultrastructural remodeling.

Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal nanoscale details of mitochondrial fission in mammalian cells, showing coordinated membrane constriction and the role of actin and septin filaments.

Outcomes of the 2019 EMDataResource model challenge: validation of cryo-EM models at near-atomic resolution

The paper evaluates cryo-EM model quality using benchmark maps, showing high accuracy and reproducibility across 13 teams and recommending multiple metrics for model assessment.

Differences in the chitinolytic activity of mammalian chitinases on soluble and crystalline substrates

This study identifies mutations that enhance chitinase activity on oligomeric substrates but not crystalline chitin, highlighting the importance of using physiologically relevant substrates in enzyme engineering.

Temperature-Jump Solution X-ray Scattering Reveals Distinct Motions in a Dynamic Enzyme

This study uses temperature-jump X-ray scattering to analyze dynamic motions in cyclophilin A, revealing two distinct relaxation processes linked to substrate specificity and catalytic turnover.