Exon Nomenclature and Classification of Transcripts (ENACT): Systematic framework to annotate exon attributes

Abstract

AbstractO_ST_ABSMotivationC_ST_ABSIsoform diversity is known to enhance a genes functional repertoire. Despite studies on transcriptome diversifying processes (Alternate splicing/transcription), their extent and correlated impact on proteome diversity remains rudimentarily understood. ResultsThe current study presents an innovative framework, "Exon Nomenclature and Annotation of Transcripts," that centralizes exonic loci while integrating protein sequence per entity with tracking and assessing splice site variability. The resulting annotation from framework enables exon features to be tractable, facilitating a systematic analysis of isoform diversity. Our findings and case studies unveil systemic exon inclusions roles in regulating diversity in CDS region. Availability and implementationAll data generated during this study are publicly available at www.iscbglab.in/enactdb/. Associated algorithmic procedures have been described in the methods section. Supplementary informationPDF file enclosing supplementary data attached.