Phototropin modulates biosynthetic gene clusters network mediated biomanufacturing of anti-inflammatory metabolites in green lineage simply by illumination

Abstract

Light sensing proteins, photoreceptor, coordinate with photosynthetic machinery, influencing both photosynthetic efficiency and several metabolic outcomes. This work deals with bottleneck issue of astaxanthin production from Chlamydomonas reinhardtii. We report a non-genetic, illumination-based strategy for biomanufacturing of astaxanthin under optimised blue light illumination in C. reinhardtii. Notably, first time ever, we identified and report astaxanthin synthesis-associated BGC in C. reinhardtii using plantiSMASH analysis. We discover experimental evidences for phototropin-modulated biosynthetic gene clusters (BGC)-mediated metabolite production in C. reinhardtii. We established that the prolonged exposure to fine-tuned blue light illumination substantially enhances the biomanufacturing of astaxanthin (1.6 times compared to red light) and pigments via the phototropin. It suggests that fine-tuned illumination conditions modulate molecular components in specific metabolite production. We are providing biochemical, genetic, transcriptomic, quantitative proteomics and systems biology evidences for opto-biomanufacturing of bioactive from green lineage via modulation of phototropin network with artificial illumination (without any genetic modification). By integrating data-driven analytics and systems biology-based computational pipeline, we elucidate the functional crosstalk between biosynthetic gene clusters with the phototropin and carotenoid metabolism synthesis pathway in the green lineage (terrestrial alga and higher plants). Thus, highlighting a new opto-biotechnology approach in metabolite production from various phototrophic organisms. These results establish a new avenue for opto-biomanufacturing strategies to generate bioactive molecules from microorganisms and other green lineages simply by illumination. Highlights O_LIOpto-biomanufacturing of astaxanthin in C. reinhardtii is achieved simply by illumination. C_LIO_LIBiosynthetic gene clusters of metabolite production are also controlled by light. C_LIO_LIPhotoreceptor-based opto-biomanufacturing of valuable bioactives is established. C_LIO_LIOpto-biomanufacturing opens avenues for bioactive production in various organisms. C_LI