Reducing Honey Bee Winter Mortality with Molybdenum Supplementation: Field Evidence Across Europe

Nutrition is key to improve honey bees resilience to environmental stress factors that threaten their health. Yet, little is known about the micronutritional needs of colonies, in particular about molybdenum, an essential trace element in biology. This study focuses on a coordination complex based on molybdenum Mo(+V) which was assessed for compatibility with standard beekeeping practices. It was found to be non-toxic for bees, stable and easy to use. Importantly, it does not leave any residues in honey produced. This study investigated how supplementation with few milligrams of this complex can improve hive performance at early spring and reduce winter mortality. Over a two-year field campaign, 283 beehives spread over 6 apiaries located in Spain, Greece and France were involved: 142 beehives supplemented with the molybdenum-based compound and 141 beehives as a control group. Supplementation resulted in a significant reduction in winter colony losses, averaging a 44% decrease, ranging between 27% and 75% in the different apiaries. The risk of death was twice lower in the Na-Mo2O4-EDTA group compared to the control group (risk ratio: 0.51). The impact on bee populations and honey production was also evaluated. A significant increase in honey reserves within the brood area of 107% was observed in the Greek apiary, whereas no comparable effect was detected in Spain, suggesting that local environmental conditions or management practices may influence this parameter. This study highlights the importance of molybdenum in the management of honey bees as an efficient tool to reduce the winter mortality of the colonies. HighlightsO_LIMolybdenum-based feed supplementation reduced honey bee winter mortality by 44% in average and up to 75% C_LIO_LIThere are no adverse effects on honey bee health or honey and wax composition. C_LIO_LISupplement showed variable effects on honey reserves and no effects on bee population. C_LIO_LIALP levels increased transiently, while the TAC was unaffected.Supplementation with Molybdenum-based compound represents a promising strategy to enhance colony overwintering success C_LI