Possible Regulation of Toll-Like Receptor 4 By Lysine Acetylation Through LPCAT2 Activity in RAW264.7 Cells.

1Inflammation is central to several diseases. TLR4 mediates inflammatory signals, however, there are gaps in the understanding of its mechanisms. Recently, TLR4 was found to co-localise with LPCAT2, a lysophospholipid acetyltransferase. This interaction influenced TLR4 subcellular localisation through an unknown mechanism. In this study, we have combined computational analysis, RNA interference technology, and biochemical analysis to investigate the possibility of TLR4 lysine acetylation and the influence of LPCAT2 on the detected lysine acetylation. The results suggest for the first time that TLR4 can undergo lysine acetylation and LPCAT2 can influence TLR4 lysine acetylation. This lays a foundation for further research on the role of lysine acetylation on TLR4 and characterisation of LPCAT2 as a protein acetyltransferase.