Advanced PCL-Chitosan Nanofibrous Wound Care Material for Enhanced Wound Healing

Abstract

Wet electrospinning has many advantages over conventional electrospinning processes. However, the technique is not commonly used due to the limited availability of suitable materials and accessibility of the technique. In this study different ratios of PCL-chitosan blend was prepared and fabricated into a highly porous scaffold system by wet electrospinning. The keratinocytes and fibroblast cells were seeded on the top and bottom side of the scaffold respectively to develop a bilayer skin construct. We conducted tests to examine the biological properties of the material including their cell viability, in vitro wound healing efficiency, and gene expression of collagen-I, collagen-III, cytokeratin-14, and cytokeratin-5. The findings indicate that PCL-chitosan can serve as a highly effective wound dressing material, with remarkable wound healing properties.