Rapid, Low-Cost Detection of Water Contaminants Using Regulated In Vitro Transcription
Jaeyoung Kirsten Jung, Khalid K. Alam, Matthew S. Verosloff, Daiana A. Capdevila, Morgane Desmau, Phillip R. Clauer, Jeong Wook Lee, Peter Quoc Nguyen, Pablo Pastén, Sandrine J. Matiasek, Jean‐François Gaillard, David P. Giedroc, James J. Collins, Julius B. Lucks
A cell-free platform pairing T7 RNA polymerase, allosteric transcription factors and DNA templates yields fluorescent RNA output to detect antibiotics, small molecules and metals in water.
Nature Biotechnology · Jul 6, 2020Peer-reviewedSynthetic Biology✦ In-depth digest