Khalid K. Alam

The paper develops and optimizes a one-pot NASBA-Cas13a assay for rapid, sensitive detection of SARS-CoV-2 RNA without specialized equipment.

FASTAptameR 2.0 provides a user-friendly web tool for analyzing combinatorial sequence selections with improved performance and features.

This paper develops DNA strand displacement circuits to enhance biosensors by enabling fine-tuning of reaction kinetics and faster response times, creating binary outputs that encode target molecule concentrations.

A cell-free platform pairing T7 RNA polymerase, allosteric transcription factors and DNA templates yields fluorescent RNA output to detect antibiotics, small molecules and metals in water.

This paper designs a cell-free biosensor for atrazine detection using a metabolic pathway and a cyanuric acid biosensor, achieving faster and tunable detection compared to whole-cell sensors.

This paper designs a portable biosensor for detecting cyanuric acid using a hybrid promoter system in E. coli, enabling rapid and on-demand detection with fluorescent and colorimetric reporters.

The paper identifies broad-spectrum RNA aptamers that inhibit diverse primate lentiviral reverse transcriptases using a Poly-Target selection approach.

This paper introduces a split-aptamer system called Split-Broccoli that enables visualization of RNA-RNA assembly in vivo through fluorescence activation upon hybridization of two RNA strands.