Image quality metrics fail to accurately represent biological information in fluorescence microscopy
Ihuan Gunawan, Richard J. Marsh, Nandini Aggarwal, Erik H. W. Meijering, Susan Cox, John George Lock, Siân Culley
The paper shows that image quality metrics (IQMs) are unreliable for guiding fluorescence microscopy image processing as they do not correlate with downstream biological analysis performance.
bioRxiv · Aug 5, 2025 · PreprintBioinformatics