Érika Endo Kokubun

The paper profiles T cell receptor alpha repertoires in IBD patients across multiple cohorts, identifies disease-associated clonotypes, and validates findings in an independent dataset.

This study evaluates how methodological choices in bulk TCR-Seq affect T cell repertoire profiling, finding that RNA/DNA quality significantly impacts results and that low quantities can be partially compensated by increasing PCR cycles.

The paper identifies and validates T cell receptor clonotypes associated with Crohn's disease and ulcerative colitis, suggesting they are potential therapeutic targets.