Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes
T. Zhou, I.-T. Teng, A. S. Olia, G. Cerutti, J. Gorman, A. Nazzari, W. Shi, Y. Tsybovsky, L. Wang, S. Wang, B. Zhang, Y. Zhang, P. S. Katsamba, Y. Petrova, B. B. Banach, , P. D. Kwong
The paper develops biotin-labeled SARS-CoV-2 spike molecular probes using structure-based design and purification methods, achieving high yields and enabling antibody characterization.
bioRxiv · Jun 22, 2020Immunology