B. J. DeKosky

Immortalization and Functional Screening of Natively Paired Human T Cell Receptor Repertoires

The paper develops a high-throughput method to functionally evaluate natively paired human T cell receptors against multiple pMHCs, validated through affinity-based mapping and sequencing.

Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron

The paper designs and produces molecular probes for SARS-CoV-2 variants to assess immune responses and isolate neutralizing antibodies.

Regulatory approved monoclonal antibodies contain framework mutations predicted from human antibody repertoires

The paper predicts framework mutations in therapeutic monoclonal antibodies using natural human antibody repertoires, showing potential to improve antibody properties and reduce immunogenicity.

Human antibody immune responses are personalized by selective removal of MHC-II peptide epitopes

The paper shows that MHC-II epitope removal from BCRs correlates with antibody development, T cell help, and long-term serum antibody secretion.

Paired heavy and light chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses

This study identifies a public antibody class, IGHV3-53/3-66, with potent SARS-CoV-2 neutralization capabilities through paired heavy and light chain interactions.

Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes

The paper develops biotin-labeled SARS-CoV-2 spike molecular probes using structure-based design and purification methods, achieving high yields and enabling antibody characterization.