Characterization of Streptococcus uberis Cas9 (SuCas9) - a Type II-A Ortholog Functional in Human Cells
Aleksandra Vasileva, Marina Abramova, Polina Selkova, A. N. Arseniev, Olga S. Musharova, Polina V Malysheva, Alina Demkina, Mikhail A. Khodorkovskii, Konstantin Severinov
This study characterizes SuCas9, a compact Cas9 ortholog from Streptococcus uberis, showing it can cleave DNA in vitro and function in human cells with a novel PAM sequence.
The CRISPR Journal · Dec 30, 2025Peer-reviewedMolecular Biology