Marina Abramova

This paper introduces an active learning method to discover PAMs for uncharacterized CRISPR-Cas9 orthologs without sequencing, reducing data requirements and validating it on known and novel Cas9 variants.

This study characterizes SuCas9, a compact Cas9 ortholog from Streptococcus uberis, showing it can cleave DNA in vitro and function in human cells with a novel PAM sequence.

The paper describes modifications to the CRISPR-Cas12d system, identifies catalytically inactive variants, and demonstrates their high specificity using single-molecule techniques.