Author

Polina V Malysheva

ITMO UniversityORCID 0009-0004-3294-9349Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 1 Molecular Biology· 1 Systems Biology· 1

Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 1 Research Focus:benchmarking· 1 Approach:experimental assay· 1 Research Focus:GWAS / association mapping· 1 Assay:GWAS summary statistics· 1 Organism:human· 1 Approach:machine learning (classical)· 1 Organism:mouse· 1

Frequent Co-Authors

Aleksandra VasilevaiD· 2 shared Alina Demkina· 2 shared Konstantin SeverinoviD· 2 shared Marina AbramovaiD· 2 shared Mikhail A. KhodorkovskiiiD· 2 shared A. N. Arseniev· 1 shared Aleksandra Andreevna Gorkovskaia· 1 shared Alexander V. PredeusiD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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2 shared papersBioengineering

Aleksandra VasilevaORCID

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Author profile →

3 papers

Recent·Most cited

Sequencing-Free Protospacer-Adjacent Motif Discovery for Uncharacterized CRISPR-Cas9 Orthologs via Active Learning

Bogdan Kirillov, Aleksandra Vasileva, O. Fedorov, Yuliya Kuz'mina, Matvey Kolesnik, Anna B. Trofimova, Alina Demkina, Viktoriia Ronchikova, Mariya Drozhzhina, Marina Abramova, Aleksandra Andreevna Gorkovskaia, Polina V Malysheva, Katherine Vilinski-Mazur, Mikhail A. Khodorkovskii, Maxim Panov, Konstantin Severinov

This paper introduces an active learning method to discover PAMs for uncharacterized CRISPR-Cas9 orthologs without sequencing, reducing data requirements and validating it on known and novel Cas9 variants.

bioRxiv · Oct 21, 2026 · PreprintBioengineering

Characterization of Streptococcus uberis Cas9 (SuCas9) - a Type II-A Ortholog Functional in Human Cells

Aleksandra Vasileva, Marina Abramova, Polina Selkova, A. N. Arseniev, Olga S. Musharova, Polina V Malysheva, Alina Demkina, Mikhail A. Khodorkovskii, Konstantin Severinov

This study characterizes SuCas9, a compact Cas9 ortholog from Streptococcus uberis, showing it can cleave DNA in vitro and function in human cells with a novel PAM sequence.

The CRISPR Journal · Dec 30, 2025Peer-reviewedMolecular Biology

Functional determinants and evolutionary consequences of pleiotropy in complex and Mendelian traits

Yury A. Barbitoff, Поліна Богайчук, Nadezhda S. Pavlova, Polina V Malysheva, Alexander V. Predeus

This study investigates pleiotropy across species and trait types, finding common features and selection pressures in highly pleiotropic genes.

Molecular Biology and Evolution · Sep 17, 2025Peer-reviewedSystems Biology