Cell-based assay to determine Type 3 Secretion System translocon assembly in Pseudomonas aeruginosa using split luciferase
H. Guo, E. J. Geddes, T. J. Opperman, A. P. Heuck
The paper describes a cell-based assay using split luciferase to detect T3SS translocon assembly in Pseudomonas aeruginosa, showing high signal/noise ratio and robustness for inhibitor screening.
bioRxiv · Jun 22, 2023 · PreprintMicrobiology