Optimised Nanobody-based Quenchbodies for Enhanced Protein Detection
J. H. Cater, N. Elsalamouny, G. H. Mansour, S. Hutchinson, C. Mc Guinness, S. H. Mueller, R. R. Spinks, N. Shanmugam, A. Pichard-Kostuch, V. Zahoransky, H. Ghodke, M. Ribezzi-Crivellari, H. Yu, A. M. van Oijen, A. D. Griffiths, L. M. Spenkelink
This study optimizes nanobody-based quenchbodies for enhanced protein detection, achieving fluorescence increases of 1.5-2.4-fold and detecting IL-6 down to 2 nM.
bioRxiv · Mar 27, 2024Bioengineering