Author

L. M. Spenkelink

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 3 Biochemistry· 2

Signature Assays, Organisms & Methods

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Organism:E. coli· 4 Approach:experimental assay· 4 Research Focus:cell signaling & trafficking· 3 Research Focus:chromatin & epigenetic regulation· 2 Approach:microscopy & imaging analysis· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1 Assay:DNA methylation· 1

Frequent Co-Authors

A. M. van Oijen· 5 shared J. S. Lewis· 3 shared N. E. Dixon· 3 shared S. Jergic· 3 shared S. H. Mueller· 2 shared Z.-Q. Xu· 2 shared A. Robinson· 1 shared A. Shirbini· 1 shared

Activity by Year

2018-2022 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 26 mutual co-author links

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5 shared papersBiophysics

A. M. van Oijen

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5 papers

Recent·Most cited

Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase

K. S. Whinn, Z.-Q. Xu, S. Jergic, N. Sharma, L. M. Spenkelink, N. E. Dixon, A. M. van Oijen, H. Ghodke

This study visualizes how the Escherichia coli Rep helicase rescues stalled replication forks by removing roadblocks, showing replication restart is the rate-limiting step.

bioRxiv · Dec 4, 2022 · PreprintBiochemistry

Rapid single-molecule characterisation of nucleic-acid enzymes

S. H. Mueller, L. J. Fitschen, A. Shirbini, S. M. Hamdan, L. M. Spenkelink, A. M. van Oijen

This paper introduces a single-molecule assay to rapidly characterize DNA metabolism enzymes by measuring fluorescence intensity changes during DNA synthesis, unwinding, or digestion.

bioRxiv · Mar 3, 2022Biophysics

The E. coli helicase does not use ATP during replication.

L. M. Spenkelink, R. R. Spinks, S. Jergic, J. S. Lewis, N. E. Dixon, A. M. van Oijen

The paper shows that the E. coli DnaB helicase does not use ATP during DNA replication, with polymerase-driven nucleotide incorporation providing the energy instead.

bioRxiv · Jul 7, 2021 · PreprintBiochemistry

Tunability of DNA polymerase stability during eukaryotic DNA replication

J. S. Lewis, L. M. Spenkelink, G. D. Schauer, O. Yurieva, S. H. Mueller, V. Natarajan, G. Kaur, C. Maher, C. Kay, M. E. O'Donnell, A. M. van Oijen

The paper shows how DNA polymerase stability and exchange dynamics in the yeast replisome are tuned by local concentrations, enabling efficient replication and adaptation to genomic challenges.

bioRxiv · Jun 7, 2019Biophysics

Recycling of single-stranded DNA-binding protein by the bacterial replisome.

L. M. Spenkelink, J. S. Lewis, S. Jergic, Z.-Q. Xu, A. Robinson, N. E. Dixon, A. M. van Oijen

This study visualizes how the E. coli replisome recruits and recycles single-stranded DNA-binding protein using two mechanisms, showing their physiological relevance.

bioRxiv · Dec 4, 2018Biophysics