Single-cell quantification of the concentration and dissociation constant of endogenous proteins
A. T. Komatsubara, Y. Goto, Y. Kondo, M. Matsuda, K. Aoki
The paper quantifies endogenous protein concentration and dissociation constant in living cells using CRISPR/Cas9 and FCCS, revealing dynamic changes in protein interactions.
bioRxiv · Nov 1, 2018Biochemistry