Engineered Antigen-Binding Fragments for Enhanced Crystallisation of Antibody:Antigen Complexes
H. A. Bruce, A. U. Singer, E. V. Filippova, L. L. Blazer, J. J. Adams, L. Enderle, M. Ben-David, E. H. Radley, D. Y. Mao, V. Pau, S. Orlicky, F. Sicheri, I. Kourinov, S. Atwell, A. A. Kossiakoff, S. S. Sidhu
This study identifies surface substitutions that enhance the crystallisation of Fab:antigen complexes by combining surface entropy reduction with phage-display technology and previously reported substitutions.
bioRxiv · Jul 6, 2023 · PreprintBiochemistry