A. A. Kossiakoff

Structural analysis and conformational dynamics of a holo-adhesion GPCR reveal interplay between extracellular and transmembrane domains

This study uses cryo-EM and single-molecule FRET to show conformational coupling between the extracellular and transmembrane domains of an aGPCR, revealing how ECR dynamics influence receptor function.

Structure of hepatitis B/D antiviral drug Bulevirtide bound to its receptor protein NTCP

The paper determines the cryo-EM structure of bulevirtide bound to NTCP, revealing its mechanism of inhibiting HBV/HDV entry and providing insights for drug development.

Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme binding

The paper determines the cryo-EM structure of P. aeruginosa PhuR bound to heme, revealing the role of Y529 in iron coordination and providing insights for developing conformation-specific antibodies.

ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding

This paper reports cryo-EM structures of ACKR3-arrestin2/3 complexes phosphorylated by GRK2 or GRK5, revealing how phosphorylation barcodes influence arrestin binding modes and receptor configuration.

Engineered Antigen-Binding Fragments for Enhanced Crystallisation of Antibody:Antigen Complexes

This study identifies surface substitutions that enhance the crystallisation of Fab:antigen complexes by combining surface entropy reduction with phage-display technology and previously reported substitutions.

Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin

This paper uses cryo-EM to determine the structure of a synthetic antibody complex with claudin-4 and CpE, revealing binding mechanisms and subtype selectivity.

Conformation-specific synthetic antibodies discriminate multiple functional states of the ion channel CorA

This study uses synthetic antibodies to identify and characterize different conformational states of the CorA ion channel under varying magnesium conditions.

Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder

This study develops an antibody that specifically modulates ADGRL3 function without affecting ADGRL1, demonstrating isoform- and ligand-specific aGPCR modulation.

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels

This study determines the structure of LRRC8A:C channels and shows how lipid interactions regulate their gating and assembly.

CryoEM Structures of the Human HIV-1 Restriction Factor SERINC3 and Function as a Lipid Transporter

The paper shows SERINC3 has a structure similar to lipid transporters and demonstrates its lipid flipping activity, which correlates with reduced HIV-1 infectivity.

Development, Structure, and Mechanism of Synthetic Antibodies that Target Claudin and Clostridium perfringens Enterotoxin Complexes

This paper develops synthetic antibodies (sFabs) targeting claudin-4 and Clostridium perfringens enterotoxin (CpE) complexes, revealing their binding mechanisms and structural insights to inform therapeutic strategies.

Architecture of the cytoplasmic face of the nuclear pore

The paper establishes the near-atomic molecular architecture of the cytoplasmic face of the human nuclear pore complex using interdisciplinary methods.

Quaternary structure independent folding of voltage-gated ion channel pore domain subunits

This paper shows that voltage-gated ion channel pore domains can maintain their structure without relying on quaternary interactions, revealing new insights into their folding and assembly.

Targeting a proteolytic neo-epitope of CUB-domain containing protein 1 in RAS-driven cancer

The paper develops antibodies targeting proteolytic neo-epitopes of CDCP1 in RAS-driven cancers, showing improved tumor specificity and safety.

K29-Linked Ubiquitin Signaling Regulates Proteotoxic Stress Response and Cell Cycle

This study identifies sAB-K29, a tool to detect K29-linked polyubiquitin, and shows its role in proteotoxic stress response and cell cycle regulation.