S. S. Sidhu

Structural and biophysical characterisation of ubiquitin variants that specifically inhibit the ubiquitin conjugating enzyme Ube2d2

The paper identifies ubiquitin variants that inhibit Ube2d2, showing structural and biophysical properties that suggest potential for developing selective E2 enzyme inhibitors.

Engineered Antigen-Binding Fragments for Enhanced Crystallisation of Antibody:Antigen Complexes

This study identifies surface substitutions that enhance the crystallisation of Fab:antigen complexes by combining surface entropy reduction with phage-display technology and previously reported substitutions.

Activity-based profiling of cullin-RING ligase networks by conformation-specific probes

The paper develops conformation-specific probes to profile activated cullin-RING ligase networks, revealing differential regulation across cell types and activation pathways.

Structural surfaceomics reveals an AML-specific conformation of Integrin-β2 as a CAR-T therapy target

The paper identifies an AML-specific conformation of Integrin-β2 using structural surfaceomics and demonstrates its targeting by CAR-T cells.

In vivo CRISPR screens reveal SCAF1 and USP15 as novel drivers of pancreatic cancer

This study uses in vivo CRISPR screens to identify USP15 and SCAF1 as novel tumor suppressors in pancreatic cancer, with potential therapeutic implications.

Ultrapotent and Broad Neutralization of SARS-CoV-2 Variants by Modular, Tetravalent, Bi-paratopic Antibodies

This study develops a tetravalent antibody format that neutralizes SARS-CoV-2 variants with high potency and breadth, surpassing conventional bivalent antibodies.

Multifaceted N-degron recognition and ubiquitylation by GID/CTLH E3 ligases

The paper identifies novel N-terminal motifs recognized by Gid4 and Gid10 E3 ligases and shows how structural flexibility and substrate context influence ubiquitylation efficiency.

Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling

This study determines the structure of ROR2's CRD and Kr domains, revealing a novel mechanism for WNT5A signaling and identifying the CRD's role in ROR2 function and secretion.

Identification of ubiquitin variants that inhibit the E2 ubiquitin conjugating enzyme, Ube2k

The paper identifies ubiquitin variants that inhibit Ube2k by binding to a hydrophobic cleft, blocking ubiquitin transfer and enzyme activation.

Generation of a New Frizzled 2 Flox Mouse Model to Clarify Its Role in Development

The paper generates a new Fzd2 flox mouse model to investigate tissue-specific roles of Fzd2, resolving contradictions in previous global knockout studies.

Multi-antigen recognition circuits overcome challenges of specificity, heterogeneity, and durability in T cell therapy for glioblastoma

This study develops multiantigen recognition circuits for CAR T cells to improve specificity, heterogeneity, and durability in glioblastoma treatment.

Synthetic antibodies neutralize SARS-CoV-2 infection of mammalian cells

The paper describes synthetic monoclonal antibodies that neutralize SARS-CoV-2 by binding to the spike protein and blocking ACE2 receptor interaction, showing potent antiviral activity.

Moyamoya Disease-Associated RNF213 Alleles Encode Dominant Negative Alleles That Globally Impair Ubiquitylation

This study identifies MMD-associated RNF213 SNPs as dominant negative alleles that impair ubiquitylation, suggesting reduced E3-ligase activity as central to MMD pathogenesis.

Protein structure from experimental evolution

This study uses laboratory evolution to infer protein structures by analyzing residue interactions from evolved sequences.

A Protein Engineering Approach for Uncovering Cryptic Ubiquitin-binding Sites: from a Ubiquitin-Variant Inhibitor of APC/C to K48 Chain Binding

The paper identifies a cryptic ubiquitin-binding site on APC/C using ubiquitin variants, revealing its preference for K48-linked chains and demonstrating a method for uncovering such sites in large complexes.