Structure-optimized sgRNA selection with PlatinumCRISPr for efficient Cas9 generation of knock-outs
Irmgard U. Haussmann, Thomas C. Dix, David W. J. McQuarrie, Veronica Dezi, Abdullah I. Hans, Roland R. Arnold, Matthias Soller
The paper develops PlatinumCRISPr, a tool for selecting efficient sgRNAs for CRISPR-Cas9 genome editing, identifies design constraints, and reports unintended DNA insertions from deletions.
bioRxiv · Dec 15, 2022 · PreprintMolecular Biology